recombinant mouse cd14 fc chimera Search Results


mouse cd14  (R&D Systems)
90
R&D Systems mouse cd14
FIGURE 6. rCRT/39–272 activates murine B cells and macrophages through the <t>CD14/TLR4</t> pathway. Freshly purified splenic B cells (4 3 105 cells/ well) from C3H/HeN and C3H/HeJ mice were stimulated, in triplicates, with rCRT/39–272 at various concentrations or with LPS (3 mg/ml) in 96-well plates for 72 h. During the last 8 h of culture, the cells were pulsed with [3H]TdR, and the results are expressed as [3H]TdR incorporation (cpm) 6 SD (A). Freshly prepared peritoneal macrophages (1 3 105 cells/well) from C3H/HeN and C3H/HeJ mice were also stimulated with rCRT/39–272 (0.3–10 mg/ml), LPS (3 mg/ml), or peptidoglycan (20 mg/ml) in 96-well plates for 48 h. Concentration of TNF-a in the culture supernatant was determined using an ELISA kit, and the results are expressed as mean 6 SD (B). Modified CRT-based ELISA was performed for identification of CRT-specific ligands (C), in which plate-bound rCRT/39–272 was allowed to react with recombinant soluble fusion protein mCD14-hIgG1-Fc (rmCD14), mCD40-hIgG1-Fc (rmCD40), or mBAFFR-hIgG1-Fc (rmBAFFR) in triplicate wells for 1 h, followed by HRP-conjugated goat anti-human IgG with OPD as substrate. The results are expressed as mean OD492 6 SD. pp , 0.05; ppp , 0.01. As additional specificity controls, LPS-based (D) and CD40L-based (E) ELISAs were also carried out in similar fashion. Results are representatives of three independent experiments. PGN, peptidoglycan.
Mouse Cd14, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+cd14+fc+chimera/Recombinant+Mouse+CD14+Fc+Chimera+Protein%2C+CF/pm20855873-57-8-33
Average 90 stars, based on 1 article reviews
mouse cd14 - by Bioz Stars, 2026-10
90/100 stars
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recombinant mouse cd14 fc chimera protein  (R&D Systems)
90
R&D Systems recombinant mouse cd14 fc chimera protein
FIGURE 6. rCRT/39–272 activates murine B cells and macrophages through the <t>CD14/TLR4</t> pathway. Freshly purified splenic B cells (4 3 105 cells/ well) from C3H/HeN and C3H/HeJ mice were stimulated, in triplicates, with rCRT/39–272 at various concentrations or with LPS (3 mg/ml) in 96-well plates for 72 h. During the last 8 h of culture, the cells were pulsed with [3H]TdR, and the results are expressed as [3H]TdR incorporation (cpm) 6 SD (A). Freshly prepared peritoneal macrophages (1 3 105 cells/well) from C3H/HeN and C3H/HeJ mice were also stimulated with rCRT/39–272 (0.3–10 mg/ml), LPS (3 mg/ml), or peptidoglycan (20 mg/ml) in 96-well plates for 48 h. Concentration of TNF-a in the culture supernatant was determined using an ELISA kit, and the results are expressed as mean 6 SD (B). Modified CRT-based ELISA was performed for identification of CRT-specific ligands (C), in which plate-bound rCRT/39–272 was allowed to react with recombinant soluble fusion protein mCD14-hIgG1-Fc (rmCD14), mCD40-hIgG1-Fc (rmCD40), or mBAFFR-hIgG1-Fc (rmBAFFR) in triplicate wells for 1 h, followed by HRP-conjugated goat anti-human IgG with OPD as substrate. The results are expressed as mean OD492 6 SD. pp , 0.05; ppp , 0.01. As additional specificity controls, LPS-based (D) and CD40L-based (E) ELISAs were also carried out in similar fashion. Results are representatives of three independent experiments. PGN, peptidoglycan.
Recombinant Mouse Cd14 Fc Chimera Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+cd14+fc+chimera/Recombinant+Mouse+CD14+Fc+Chimera+Protein%2C+CF/pmc01626529-549-13-25
Average 90 stars, based on 1 article reviews
recombinant mouse cd14 fc chimera protein - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
recombinant mouse cd14 fc chimera protein cf  (R&D Systems)
N/A
The Recombinant Mouse CD14 Fc Chimera Protein from R D Systems is derived from NS0 The Recombinant Mouse CD14 Fc Chimera Protein has been validated for the following applications Bioactivity
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FIGURE 6. rCRT/39–272 activates murine B cells and macrophages through the CD14/TLR4 pathway. Freshly purified splenic B cells (4 3 105 cells/ well) from C3H/HeN and C3H/HeJ mice were stimulated, in triplicates, with rCRT/39–272 at various concentrations or with LPS (3 mg/ml) in 96-well plates for 72 h. During the last 8 h of culture, the cells were pulsed with [3H]TdR, and the results are expressed as [3H]TdR incorporation (cpm) 6 SD (A). Freshly prepared peritoneal macrophages (1 3 105 cells/well) from C3H/HeN and C3H/HeJ mice were also stimulated with rCRT/39–272 (0.3–10 mg/ml), LPS (3 mg/ml), or peptidoglycan (20 mg/ml) in 96-well plates for 48 h. Concentration of TNF-a in the culture supernatant was determined using an ELISA kit, and the results are expressed as mean 6 SD (B). Modified CRT-based ELISA was performed for identification of CRT-specific ligands (C), in which plate-bound rCRT/39–272 was allowed to react with recombinant soluble fusion protein mCD14-hIgG1-Fc (rmCD14), mCD40-hIgG1-Fc (rmCD40), or mBAFFR-hIgG1-Fc (rmBAFFR) in triplicate wells for 1 h, followed by HRP-conjugated goat anti-human IgG with OPD as substrate. The results are expressed as mean OD492 6 SD. pp , 0.05; ppp , 0.01. As additional specificity controls, LPS-based (D) and CD40L-based (E) ELISAs were also carried out in similar fashion. Results are representatives of three independent experiments. PGN, peptidoglycan.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Functional analysis of recombinant calreticulin fragment 39-272: implications for immunobiological activities of calreticulin in health and disease.

doi: 10.4049/jimmunol.1000536

Figure Lengend Snippet: FIGURE 6. rCRT/39–272 activates murine B cells and macrophages through the CD14/TLR4 pathway. Freshly purified splenic B cells (4 3 105 cells/ well) from C3H/HeN and C3H/HeJ mice were stimulated, in triplicates, with rCRT/39–272 at various concentrations or with LPS (3 mg/ml) in 96-well plates for 72 h. During the last 8 h of culture, the cells were pulsed with [3H]TdR, and the results are expressed as [3H]TdR incorporation (cpm) 6 SD (A). Freshly prepared peritoneal macrophages (1 3 105 cells/well) from C3H/HeN and C3H/HeJ mice were also stimulated with rCRT/39–272 (0.3–10 mg/ml), LPS (3 mg/ml), or peptidoglycan (20 mg/ml) in 96-well plates for 48 h. Concentration of TNF-a in the culture supernatant was determined using an ELISA kit, and the results are expressed as mean 6 SD (B). Modified CRT-based ELISA was performed for identification of CRT-specific ligands (C), in which plate-bound rCRT/39–272 was allowed to react with recombinant soluble fusion protein mCD14-hIgG1-Fc (rmCD14), mCD40-hIgG1-Fc (rmCD40), or mBAFFR-hIgG1-Fc (rmBAFFR) in triplicate wells for 1 h, followed by HRP-conjugated goat anti-human IgG with OPD as substrate. The results are expressed as mean OD492 6 SD. pp , 0.05; ppp , 0.01. As additional specificity controls, LPS-based (D) and CD40L-based (E) ELISAs were also carried out in similar fashion. Results are representatives of three independent experiments. PGN, peptidoglycan.

Article Snippet: Recombinant mouse CD40L and recombinant fusion proteins between mouse CD14 and human IgG1 Fc fragment (rmCD14-Fc), mouse CD40 and human IgG1 Fc (rmCD40-Fc), mouse BAFFR and human IgG1 Fc (rmBAFFR-Fc) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Recombinant